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Electrophysiology Neurons were constantly perfused at 3 ml min −1 with heated (30–32 °C), gassed (95% O 2 , 5% CO 2 ) artificial cerebrospinal fluid (pH 7.4, 300–305 mOsm) containing (in mM): 119 NaCl, 2.5 KCl, 1.3 MgCl 2 , 2.5 CaCl 2 , 26 NaHCO 3 , 1.3 NaH 2 PO 4 , 20 D-glucose and 0.5 sodium ascorbate