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After centrifugation at 1,000 rpm at room temperature, cell pellets were washed once with 1 ml wash buffer (20 mM HEPES (pH 7.5), 150 mM NaCl, 0.5 mM spermidine (MilliporeSigma, S2501) and cOmplete, EDTA-free protease inhibitor cocktail (MilliporeSigma, 11873580001) in nuclease-free water)
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