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The offspring from dams fed with mixed choline sources with lower free choline (12.5-25%) but high glycerophosphocholine (25-75%), exhibited a decrease in macrophage and dendritic cell frequencies in the spleen and produced less IL-1β, IL-6 and IFN-γ in response to mitogenic immune challenge with either concanavalin A or LPS, compared to those from dams with higher free choline (100%) intake ( de novo PC biosynthesis ( de novo PC synthesis, accelerated PC turnover, and proinflammatory adipose tissue macrophage activation ( In dendritic cells, ACh modulates immune function by upregulating HLA-DR and CD86 expression and stimulating TNF-α and IL-8 production ( Enterobacter ludwiggi , an abundant commensal bacterial species in mice, enhances dendritic cell immune tolerance and promotes Treg differentiation via choline metabolism ( E
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Thus, although MeHg exhibits markedly weaker non-covalent docking scores, its high electrophilicity and strong thiophilicity (propensity to bind cysteine/thiol groups) mean that even transient interactions near cysteine- and aromatic-rich regions of epigenetic enzymes could plausibly induce allosteric perturbations
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