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While the method showed reduced precision in comparison to potentiometric or conductometric methods, this was offset by its faster measurement times and lower sample requirements.59 However, determining pK a values by isotachophoresis was not without disadvantages which are discussed in detail by Beckers et al.60 It was remarked that the calculations required are quite laborious as the zones formed during the separation all have different parameters (pH, ionic strength, and even temperature)

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