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For GSSG assay, 100 µL extracted sample was added to 100 µL of 40 mM N -ethylmaleimide (NEM) (1:1 ratio), incubated at 25 °C for 25 min and mixed with 250 µL 0.1 M sodium hydroxide (NaOH)
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By leveraging droplet microfluidics, entire gene libraries can be encapsulated within droplets, with each droplet containing a unique gene variant
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