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On the day of analysis, the medium was removed and the cells were washed once with 200 μL Tyrode buffer (Tyrode’s Salts [9.6 g/L], 10 mM 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid [HEPES], pH 7.4)
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A moisturizing cream helps replenish skin condition with deep hydration to improve fine lines and wrinkles.
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