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Larsen et al., 2000) to avoid the mono-ubiquitination of substrates that would otherwise shunt them to the endosomal-lysosome pathway to be degraded (Osaka et al., in vitro by an enzyme assay using the fluorogenic substrate Ub-AMC, and its activity can be effectively inhibited by the specific inhibitor LDN-57444 (Wilkinson et al., 1989
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