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For testing the direct incorporation of PC into myelin, by the time of myelination induction the cells were treated with 2 µg/ml BODIPY-labeled TopFluor PC (Avanti polar lipids, #810281), whereas control cultures were treated with 2 µg/ml BODIPY-labeled pentanoic acid (ThermoFisher, #D3834)
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The role of E3 ligases in autoimmunity and the regulation of autoreactive T cells
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