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Specifically, chondrocytes exposed to ferric ammonium citrate (FAC) exhibit increased expression of FTH1 and reduced levels of transferrin receptor 1 (TfR1) and hepcidin, whereas ferroportin (FPN1) expression remains unchanged ( 3.3.2 Fenton reactions and the bidirectional effects of iron homeostasis imbalance Free Fe² + participates in the Fenton reaction, leading to the generation of hydroxyl radicals (·OH), which represents a crucial step in initiating the lipid peroxidation chain reaction ( 3.3.3 Local iron homeostasis and the joint microenvironment Pathological changes such as increased vascular permeability or microbleeding in subchondral bone can also lead to local iron deposition