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In brief, 5x T225-flasks were triple-transfected with (i) the pHelper plasmid from the AAVpro® Helper Free System (AAV6) kit (Takara Bio Inc.), (ii) the plasmid pAAV2/8RGD containing coding sequences of the AAV2-derived rep proteins and the modified AAV8 capsid proteins and (iii) the pAAV-Cp-SV40pA derivate containing the AAV-genome with the respective transgene
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